Coding

Part:BBa_K209008:Design

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-09-11)


Aar1 C-D part, PI3k1 (-N) (Dictystelium dicoideum)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2131
    Illegal SpeI site found at 205
    Illegal PstI site found at 2279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2131
    Illegal SpeI site found at 205
    Illegal PstI site found at 2279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2131
    Illegal BamHI site found at 3132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2131
    Illegal SpeI site found at 205
    Illegal PstI site found at 2279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2131
    Illegal SpeI site found at 205
    Illegal PstI site found at 2279
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Aar1 CD part in pDONR221. BBF RFC 28.


Source

from plasmid

References